Dear LSI Imaging Users and Labs,
We’re excited to share some exciting updates that enhance our imaging capabilities and support your research projects:
New Super-Resolution Microscopes:
Our facility has just integrated three state-of-the-art super-resolution imaging systems—SIM (Structured Illumination Microscopy), dSTORM (Direct Stochastic Optical Reconstruction Microscopy), and PALM (Photoactivated Localization Microscopy). Now, we are placed among the bioimaging facilities worldwide that offer a nearly full spectrum of advanced imaging technologies, spanning wide-field, confocal, SIM, STED, SMLM covering the biological scales from mm to nm. To learn more about their capabilities please visit https://imaging.lsi.ubc.ca/instruments/.
- Wide field for large IHC, IF, H&E samples (with following example studies)
- Microfluidic platform imaged with Thunder Wide Field- Nature Communications-https://www.nature.com/articles/s41467-024-45710-4
- CL protease project imaging with wide field – Cell Reports-https://www.cell.com/cell-reports/fulltext/S2211-1247(24)01431-1
- Confocal imaging for live and fixed samples (slides, multiple plates or dishes) with up to 9 colors (with following example studies)
- Cells imaged on 96-well plates with 3D confocal –Nature-https://www.nature.com/articles/s41586-022-04661-w
- Nanosensors with 3D confocal – ACS Sensors-https://pubs.acs.org/doi/abs/10.1021/acssensors.4c00957
- Super resolution: STED (resolution ~50nm), SIM (resolution ~120nm), dSTORM (precision ~15nm), GSD (precision ~20nm), PALM (precision ~20nm) (with following example studies)
- Subcellular structures examined with 3D STED – Journal of Cell Biology-https://rupress.org/jcb/article/223/1/e202206109/276402/Membrane-contact-site-detection-MCS-DETECT-reveals
- Virus assembly studied with dSTORM- Nature Communiations-https://www.nature.com/articles/s41467-018-05480-2
- SuperResNET + SMLM/GSD + MINFLUX single-molecule network analysis – Journal of Cell Science-https://journals.biologists.com/jcs/article-abstract/138/4/JCS263570/367028/SuperResNET-single-molecule-network-analysis?redirectedFrom=fulltext
New Tissue Culture Room for Live Cell Imaging:
To better support live cell imaging projects, we are setting up a dedicated tissue culture room adjacent to the Leica Stellaris confocal and Leica SP5 II confocal. This will be particularly beneficial for projects from BCCA, hospital sites, and academic units outside the Life Sciences Centre. If you’re interested in using this facility, please contact us for details.
We look forward to seeing how these new advancements support your research. If you have any questions, please feel free to reach out!
LSI Imaging Facility